#WESTERN BLOT LADDER PROTEIN LADDER VERIFICATION#

Our Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). NIH National Institute of General Medical Sciences.Enzo's Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Penn State (Eberly College of Science Undergraduate Research Support) and We are grateful for funding support from: Choosing a protein ladder for your application Type Marker Cat. Penn State News article about this undergraduate project. Two orange reference bands at 70 kDa and 25 kDa and one green reference band at 10 kDa highlight the blue-stained protein ladder. Mixture of 10 recombinant proteins of precise molecular weights (10250 kD) 8 blue-stained bands and 2 pink reference bands (25 and 75 kD) Features and Benefits.

#WESTERN BLOT LADDER PROTEIN LADDER PLUS#

The plasmids to produce the Penn State Protein Ladders are available without licensing restrictions to nonprofit academic users through the Addgene plasmid repository: pPSU10Įxpresses 10 kD Penn State ladder proteinĮxpresses 15 kD Penn State ladder proteinĮxpresses 20 kD Penn State ladder proteinĮxpresses 30 kD Penn State ladder proteinĮxpresses 40 kD Penn State ladder proteinĮxpresses 50 kD Penn State ladder proteinĮxpresses 60 kD Penn State ladder proteinĮxpresses 80 kD Penn State ladder proteinĮxpresses 100 kD Penn State ladder proteinĮxpresses 150 kD Penn State ladder proteinĮxpresses 250 kD Penn State ladder proteinĬoexpresses 10, 30, 50, 100 kD Penn State ladder proteinsĬoexpresses 20, 40, 60, 80 kD Penn State ladder protein The PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use in protein electrophoresis (SDS-PAGE) and western blotting. Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Depending on the primary antibody, is sometimes possible to detect also the protein ladder, this does not means that your western blot is wrong or that the bands that you see are unspecific. coli and affinity protein purification, and to research laboratories desiring positive controls for recombinant protein expression and purification. The Penn State Protein Ladder expression plasmids also constitute useful reagents for teaching laboratories to demonstrate recombinant expression in E. 50 ml of culture is sufficient to produce 20,000 lanes of individual ladder protein or 3750 lanes of each set of coexpressed ladder proteins. We have also constructed plasmids to express 150 and 250 kD proteins.įor more efficient production, we have created two polycistronic expression vectors which coexpress the 10, 30, 50, 100 kD proteins or the 20, 40, 60, 80 kD proteins. Each protein migrates appropriately on SDS-PAGE gels, is expressed at very high levels (10–50 mg per liter of culture), is easy to purify via histidine tags and can be detected directly on Western blots via engineered immunoglobulin binding domains. Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots. The system includes plasmids which express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins in E. Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc. We have created the Penn State Protein Ladder system to produce protein molecular weight markers easily and inexpensively (less than a penny a lane). They provide molecular weight standards to estimate the size of protein separated by gel electrophoresis like SDS-PAGE. Protein ladders or molecular weight markers are commonly used reagents in biochemistry experiments. Make your own protein ladders for less than a penny a lane

Western blot ladder protein ladder